Potential errors in amylase experiment 1 the limits of amylase 2-1-14 abstract this report explains the purpose of this experiment in a way that conveys information to the reader about amylase’s ability to withstand acidic or basic ph. Alpha-amylase is a type of enzyme (biological catalyst) which reduces the activation energy required in the hydrolysis of starch which thus speeds up the reaction rate this reaction is increased roughly by a value of x 10¹° due to the amylase enzymes otherwise this reaction would not happen fast enough.
In this investigation, amylase is the enzyme, and starch is the substrate, ie the substance it acts upon in fact this work should add to your knowledge about the process of digestion which occurs in your body.
Enzyme experiments are ideal for “hands on” opportunities and since several factors affect the rate at which enzymatic reactions proceed, an enzyme experiment presents many opportunities in the biology laboratory the experiments presented in this chapter were amylase is an enzyme that catalyses the hydrolysis of the polysaccharide. Factors affecting the rate of enzymes activity biology essay print reference this published: 23rd march, if the same lab was done with amylase this lab would help others understand a little more in the similarities and differences between enzymes one other suggestion would be to carry out the experiments to the full potential after. The experiment was created and conducted to see how catalyst concentration affects the rate of enzyme activity the catalyst used in the experiment was amylase, which speeds up the breakdown of starch. Investigation: enzymes objectives measure the effects of changes in temperature, ph, and enzyme concentration on reaction rates of an enzyme in this lab, you will study an enzyme that is found in the cells of many living tissues pepsin is found in the stomach, amylase in the saliva, and phosphatase in the liver synthesis: how does.
Investigation: enzymes objectives measure the effects of changes in temperature, ph, and enzyme concentration on reaction rates of an enzyme pepsin is found in the stomach, amylase in the saliva, and phosphatase in the liver evidence: reasoning: part e - design an experiment lactaid is a product designed to help people who cannot. Measure the time taken for amylase to completely break down starch, the main errors will be in the order of mixing the enzyme/ substrate/ buffer, or a delay in sampling so that the reaction time is under-estimated or rate is over-estimated temperature variation affects enzyme activity, so results collected on different days are not.
The three part experiment in this lab, investigates the activity of two enzymes catalase and amylase the first and second experiment will qualitatively/ quantitatively evaluate the activity of these enzymes. Amylase is a general name for an enzyme which breaks down starch starch makes up a large proportion of our diet, and it needs to be made soluble before it can be absorbed into our bodies in order to carry out this chemical breakdown, various glands produce digestive juices containing amylases to be mixed with the food. Experimental errors it is impossible to make an exact measurement therefore, all experimental results are wrong just how wrong they are depends on the kinds of errors that were made in the experiment.
Sources of error: possible sources of human error: improper amount of ph buffer improper dilution of a-amylase solution not allowed to incubate for proper amount of time improper mixing improper amount of maltose colour reagent added solution not heated for long enough improper dilution of solution with distilled water. If the same lab was done with amylase this lab would help others understand a little more in the similarities and differences between enzymes one other suggestion would be to carry out the experiments to the full potential.
Quick answer some possible sources of errors in the lab includes instrumental or observational errors environmental errors can also occur inside the lab. D use the syringe to place 2 cm 3 of amylase into the test tube e add 1 cm 3 of buffer solution to the test tube using a syringe f use another syringe to add 2 cm 3 of starch to the amylase/ buffer solution, start the stop clock and leave it on throughout the test mix using a plastic pipette. Questions about the main experiment in this experiment, you used a plastic pot of water to keep the tubes and their contents warm what do you think would happen if you had not used it clue: think about conditions in your body slower action if cooler from your results, what can you say about the product of the action of amylase on starch ie.